Protein purification kits, HOOK™

Protein purification kits, HOOK™

For the rapid purification of soluble, 6X His or GST tagged proteins.

For bacteria or yeast cells
Includes lysis buffer and lytic enzymes
Gravity or spin format
Nickel or cobalt chelating resin for 6X His
High binding glutathione resin for GST

HOOK™ protein purification kits allow for the purification of soluble protein from bacterial and yeast cultures. The kits are supplied with a specific PE LB™ lysis buffer and lytic enzyme (LongLife™ PE LB™ Lysozyme or LongLife™ Zymolyase®) for efficient cell lysis with maintenance of the tagged protein’s properties. The 6X His tagged proteins are then purified with high binding efficiency nickel chelating or cobalt chelating resin. Cobalt chelating resin has a lower binding affinity for 6X His tags, compared to nickel chelating resin, which results in less non specific binding and may result in slightly lower yields. The GST tagged proteins are purified with a high capacity glutathione resin.

HOOK™ protein purification kits are supplied in a gravity flow format for yields of 10 mg/250 ml of culture and spin kits for rapid elution of protein for ~1 mg/50 ml of culture.

Environmentally Preferable:

Related Information

Manual

Nickel resin

Product Information

Catalog #
Supplier #
Pk
Availability
Price
HOOK™ 6X His protein purification, bacteria, 5×10 mg
HOOK™ 6X His protein purification, bacteria, 5×10 mg
786-630
786-630
1 KIT

Log in for availability
1 * 1 KIT
1 * 1 KIT - 790,00 €
790,00 €
New Customer Promotional Price
1 * 1 KIT
790,00 €
HOOK™ 6X His protein purification, yeast, 5×10 mg
HOOK™ 6X His protein purification, yeast, 5×10 mg
786-634
786-634
1 KIT

1 * 1 KIT
1 * 1 KIT - 790,00 €
790,00 €

1 * 1 KIT
790,00 €
HOOK™ 6X His protein spin purification, yeast, 25×1 mg
HOOK™ 6X His protein spin purification, yeast, 25×1 mg
786-632
786-632
1 KIT

1 * 1 KIT
1 * 1 KIT - 837,00 €
837,00 €

1 * 1 KIT
837,00 €
HOOK™ 6X His protein spin purification, bacteria, 5×1 mg
HOOK™ 6X His protein spin purification, bacteria, 5×1 mg
786-628
786-628
1 KIT

1 * 1 KIT
1 * 1 KIT - 837,00 €
837,00 €

1 * 1 KIT
837,00 €

Cobalt resin

Pk
HOOK™ 6X His protein purification, yeast, 5×10 mg
HOOK™ 6X His protein purification, yeast, 5×10 mg
786-635
786-635
1 KIT

1 * 1 KIT
1 * 1 KIT - 790,00 €
790,00 €

1 * 1 KIT
790,00 €
HOOK™ 6X His protein spin purification, yeast, 25×1 mg
HOOK™ 6X His protein spin purification, yeast, 25×1 mg
786-633
786-633
1 KIT

1 * 1 KIT
1 * 1 KIT - 837,00 €
837,00 €

1 * 1 KIT
837,00 €
HOOK™ 6X His protein purification, bacteria, 5×10 mg
HOOK™ 6X His protein purification, bacteria, 5×10 mg
786-631
786-631
1 KIT

1 * 1 KIT
1 * 1 KIT - 790,00 €
790,00 €

1 * 1 KIT
790,00 €
HOOK™ 6X His protein spin purification, bacteria, 25×1 mg
HOOK™ 6X His protein spin purification, bacteria, 25×1 mg
786-629
786-629
1 KIT

1 * 1 KIT
1 * 1 KIT - 837,00 €
837,00 €

1 * 1 KIT
837,00 €

Glutathione resin

Pk
HOOK™ GST protein purification, yeast, 5×10 mg
HOOK™ GST protein purification, yeast, 5×10 mg
786-643
786-643
1 KIT

1 * 1 KIT
1 * 1 KIT - 1 030,00 €
1 030,00 €

1 * 1 KIT
1 030,00 €
HOOK™ GST protein spin purification, yeast, 25×1 mg
HOOK™ GST protein spin purification, yeast, 25×1 mg
786-642
786-642
1 KIT

1 * 1 KIT
1 * 1 KIT - 1 030,00 €
1 030,00 €

1 * 1 KIT
1 030,00 €
HOOK™ GST protein purification, bacteria, 5×10 mg
HOOK™ GST protein purification, bacteria, 5×10 mg
786-641
786-641
1 KIT

1 * 1 KIT
1 * 1 KIT - 1 030,00 €
1 030,00 €

1 * 1 KIT
1 030,00 €
HOOK™ GST protein spin purification, bacteria, 25×1 mg
HOOK™ GST protein spin purification, bacteria, 25×1 mg
786-640
786-640
1 KIT

1 * 1 KIT
1 * 1 KIT - 1 030,00 €
1 030,00 €

1 * 1 KIT
1 030,00 €