16S Barcoding Kits deliver rapid, genus-level bacteria and archaea identification using nanopore sequencing.
- Sequence and analyse the complete 16S rRNA gene for improved taxonomic resolution
- Multiplex samples to reduce price per sample
- Analyse samples using cloud-based EPI2ME 16S pipeline or your own analysis pipeline
Barcoding or multiplexing is useful when the amount of data required per sample is less than the total amount of data that can be generated from a single flow cell: it allows a user to pool multiple samples and sequence them together making more efficient use of the flow cell.
The kit is supported by the EPI2ME 16S workflow, which can be used to analyse data from the 16S protocol. Deconvolution of barcoded sequencing data is supported by Guppy and EPI2ME which classify the barcode sequence and sort reads into corresponding folders.
The Oxford Nanopore 16S kits offer a method of amplifying and barcoding the ~1500 bp 16S rRNA gene from multiple samples and sequencing them together. By narrowing down to a specific region of interest, a user can see all the organisms in the sample without sequencing unnecessary regions of the genome, making the identification quicker and more economical. Analysing the full 16s rRNA gene has been shown to be more informative than analysing a subset of exons.
The DNA is amplified by PCR using specific 16S primers (27F and 1492R) that contain barcodes and 5’ tags which facilitate the ligase-free attachment of Rapid Sequencing Adapters.
Sample multiplexing for the 16S Barcoding Kit is ≤12, while the 16S Barcoding Kit 1-24 is ≤24.
Delivery information: Flow cells and kits are shipped together at 2 to 8 °C. Upon receipt, please place the product in a long-term storage location at ‒20 °C. Oxford Nanopore Technologies deem the useful life of the product to be three months from receipt by the customer.
Upozornení: Oxford Nanopore Technologies products are currently for research use only.