Rapid Sequencing Kits offer a simple and rapid library preparation method for genomic DNA sequencing on MinION™ devices.
- Sequence native DNA — eliminate PCR bias and retain base modifications
- Fast library prep — 10 minutes from extracted DNA
- Minimal lab equipment required
- Multiplex ≤12 samples with the Rapid Barcoding Kit
Rapid Sequencing Kits generate sequencing libraries from extracted gDNA in 10 minutes using a simple, streamlined, workflow — requiring minimal lab equipment. With no requirement for amplification, these kits eliminate potential PCR bias and preserve base modifications, which can be detected alongside the nucleotide sequence.
At the heart of these kits is a transposase which simultaneously cleaves template molecules and attaches tags to the cleaved ends. Rapid Sequencing Adapters are then added to the tagged ends.
Rapid Sequencing Kits are optimised for simplicity and speed, rather than for obtaining maximum throughput. Due to the simple nature of the workflow and the fact that little sample manipulation is required (e.g. minimal pipetting steps and no clean-ups) some very long reads can be achieved with this kit, despite the required transposase fragmentation. However, in order for long reads to be observed in sequencing, long fragments need to be present in the sample in the first place.
Barcoding or multiplexing is useful when the amount of data required per sample is less than the total amount of data that can be generated from a single flow cell: it allows a user to pool multiple samples and sequence them together, making more efficient use of the flow cell.
The Rapid Barcoding Kit attaches barcoded tags to the cleaved ends of the template molecules: there are 12 unique barcoded tags in the kit. Barcoded samples are pooled and Rapid Sequencing Adapters are then added to the tagged ends. Deconvolution of barcoded sequencing data is supported by the MinKNOW and Guppy software provided with MinION sequencing devices, which classify the barcode sequence and sort reads into corresponding folders.
For sample multiplexing of Rapid Sequencing Kit, see expansion packs. For the Rapid Barcoding Kit, it is ≤12 samples.
Delivery information: Flow cells and kits are shipped together at 2 to 8 °C. Upon receipt, please place the product in a long-term storage location at ‒20 °C. Oxford Nanopore Technologies deem the useful life of the product to be three months from receipt by the customer.
Upozornení: Oxford Nanopore Technologies products are currently for research use only.