Mutagenesis kits QuikChange

Supplier: Agilent Technologies
AGLS200518EA 1003 EUR
AGLS200518 AGLS200531 STRA200531 AGLS200519 AGLS200516 AGLS200517 AGLS200513
Mutagenesis kits QuikChange
Nucleic Acid Reagents Cloning Kits and Ligation
The original QuikChange Site-Directed Mutagenesis Kits speed up and simplify site-directed mutagenesis studies. The kits eliminate the need for sub-cloning into M13-based bacteriophage vectors and for ss-DNA rescue. This allows oligo-mediated introduction of site-specific mutations into virtually any double-stranded plasmid DNA. In addition, the XL version of the kit is specially designed for efficient mutagenesis of large (4 to 14 kb) or otherwise difficult-to mutagenise plasmid templates. The XL kit features components specifically designed for more efficient DNA replication and bacterial transformation.

  • >80% mutagenesis efficiency for one site; >55% mutagenesis efficiency for three mutations simultaneously
  • Efficient and accurate linear amplification minimises unwanted errors

QuikChange Site-Directed Mutagenesis Kit: This kit uses PfuTurbo DNA polymerase. It replicates plasmid strands with high fidelityll without displacing mutant oligonucleotide primers. The process uses a supercoiled dsDNA vector with insert and two synthetic oligo primers containing the mutation. Incorporating oligo primers creates a mutated plasmid with staggered nicks. The product is treated with Dpn I after temperature cycling. Dpn I digests the parental DNA template, selecting mutation-containing synthesised DNA. The nicked vector DNA containing the mutations is transformed into XL1-Blue supercompetent cells.

QuikChange XL Site-Directed Mutagenesis Kit: The QuikChange II XL site-directed mutagenesis kit specialises in efficient mutagenesis of large (8 to14 kb) or otherwise difficult-to mutagenise plasmid templates and features components designed for more efficient DNA replication and bacterial transformation. A QuikSolution reagent is provided to facilitate replication of large plasmids, while XL10-Gold Ultracompetent Cells have been included to ensure the highest transformation efficiencies possible. XL10-Gold cells contain the Hte phenotype, increasing the transformation efficiency of larger DNA plasmids.

QuikChange Multi site-directed mutagenesis kit: The QuikChange Multi site-directed mutagenesis system is a novel technology that allows mutagenesis at multiple sites in a single round, using a single oligonucleotide per site. This system simplifies randomising key amino acids using oligos containing degenerate codons. No specialised vectors or unique restriction sites are needed to use the kit; virtually any plasmid up to 8 kb is a suitable template. The rapid three-step procedure introduces mutations at three different sites simultaneously in the 4 kb QuikChange Multi control plasmid with >50% efficiency.

Upozornení: Academic QuikChange Multi site-directed mutagenesis Kits are for Academic Use Only.
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