Pfu DNA ligase is a recombinant enzyme derived from Pyrococcus furiosus hyperthermophilic marine archaebacterium that catalyses linkage of adjacent 5'-phosphate and 3'-hydroxy ends of double-stranded DNA at 45 to 80 °C, with a temperature optimum near 70 °C for nick-sealing reactions.
- Highly thermostable, with half-life of over 60 minutes at 95 °C
- Increases reliability of LCR by permitting higher melt temperatures
- Eliminates LCR template-predenaturation step, less enzyme required for LCR
It has higher ligation specificity and lower background than Tth DNA ligase.
Delivery information: Kit contains: 400 U Pfu DNA Ligase 10X reaction buffer
Upozornení: For research use only. Not for use in diagnostic procedures.