Gibson Assembly® HiFi HC Cloning Kits and Master Mixes

Supplier: Synthetic Genomics
SYGEGA1100-4XSEA 98.6 EUR
SYGEGA1100-4XS SYGEGA1100-4X50M SYGEGA1100-4X50 SYGEGA1100-4X10M
Gibson Assembly® HiFi HC Cloning Kits and Master Mixes
Nucleic Acid Reagents Cloning Kits and Ligation
Gibson Assembly® HiFi HC 1-Step Kits and Master Mixes allow restriction digest-free, cloning of one or more DNA fragments into virtually any location of any plasmid vector in a single round of cloning.

  • Fast and efficient: Complex assemblies with > 90% cloning efficiencies in one hour with less than five minutes hands-on time
  • Restriction digest-free: Clone without the need for restriction enzyme sites
  • Multiple inserts: Reliable assembly of up to 5 inserts at one time
  • Optimal for fragment sizes ranging from 500 bp to 32 kb
  • Concentrated 4X mix allows for use of more dilute DNA samples
  • Formulated with a thermostable proof-reading polymerase for greater numbers of error-free clones
  • Kit includes Gibson Assembly® ready positive control (also available separately)

Assemble seamless, perfect constructs in a single round of cloning using up to five inserts and fragments from 500 bp to 32 kb. Provided as a 4X master mix, the Gibson Assembly® HiFi HC 1-Step mix enables the cloning of less concentrated DNA samples. With performance identical to the original 2X Gibson Assembly® HiFi mix, the Gibson Assembly® HiFi 1-Step method allows genes to be cloned into any vector without restriction digestion. Direct cloning into the final vector avoids time consuming subcloning.

Delivery information: Shipped on dry ice. Store at −20 °C upon arrival.
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